SIBBM 2017 • Frontiers in Molecular Biology

SIBBM 2017
Frontiers in Molecular Biology

SIBBM 2017
Frontiers in
Molecular Biology

14-16 June, Milan

14-16 June

Società Italiana di Biofisica
e Biologia Molecolare
Società Italiana di Biofisica e Biologia Molecolare Società Italiana di Biofisica e Biologia Molecolare

Milan, 14-16 June 2017

From Single Cells to 3D-Cell Culture


  • Wednesday, 14 June

    12:00-13:45 Registration
    13:45-14:00 Welcome/Introductory Remarks
    Session I Tissues, Organs and Organoids
    Chairs: Marco Bianchi, Vittorio Colantuoni
    14:00-14:40 Andrea Manfrin, EPFL, Lausanne, Switzerland
    Engineering stem cells self-organization
    14:40-15:00 Short talk: Tito Panciera, University of Padua, Padua, Italy
    De novo generation somatic stem cells by YAP/TAZ
    15:00-15:20 Short talk: Elisa Lenti, San Raffaele Scientific Institute, Milan, Italy
    Development of transplantable lympho-organoids for the treatment of secondary lymphedema
    15:20-16:00 Giuseppe Testa, European Institute of Oncology (IEO), Milan, Italy
    The vulnerability of the developing human cortex to genetic and environmental causes of chromatin dysfunction: insights form 3D cortical organoids
    16:00-16:15 Gold supporter talk
    Prospero Civita, TwinHelix (IVTech)
    Drug response assay on Trastuzumab sensitive breast cancer cell lines in a dynamic 3D cell culture system
    16:15-16:30 Gold supporter talk
    Giuseppe Mancuso, Product Manager Cell Analysis & Proteomics, Promega Italia
    From 2D to 3D: a new “dimension” for cell based assays
    16:30-17:00 Coffee break
    17:00-18:15 Science & Society: Gender Equality
    Chairs: Tiziana Bonaldi, Susanna Chiocca
      dedicated to the Memory of Anna Tramontano
      Commemorative presentation by Manuela Helmer Citterich, University of Rome Tor Vergata, Rome, Italy
      Isabelle Vernos, Centre for Genomic Regulation, Barcelona, Spain
    Unifying innovative efforts of European research centres to achieve gender equality in academia (30' talk +15' discussion)
      Paolo Maugeri, European Institute of Oncology (IEO) and University of Milan, Milan, Italy
    The LIBRA project - Boosting gender equality in research (15' min talk)
    18:15-20:15 Poster Session I & Aperitif
  • Thursday, 15 June

    Session II Imaging Cells and Molecules
    Chairs: Raffaele De Francesco, Valeria Poli
    8:45-9:00 Gold supporter talk
    Giacomo Cozzi, Product Manager Research Applications Nikon Instruments Spa
    Nikon solutions for high-end microscopy
    9:00-9:40 Maria Pia Cosma, Centre for Genomic Regulation, Barcelona, Spain
    Dissecting the structure of chromatin fibres in somatic and stem cells using super resolution imaging
    9:40-10:00 Short talk: Bojana Lucic, Heidelberg University Hospital, Heidelberg, Germany
    HIV-1 targets 3D clusters of gene loci delineated with super enhancers in CD4+ T cells
    10:00-10:20 Short talk: Eelco van Anken, San Raffaele Scientific Institute, Milan, Italy
    Molecular basis of IRE1α-driven life/death decisions upon triaging the severity of endoplasmic reticulum stress
    10:20-11:00 INGM Lecture
    Ralf Bartenschlager, University of Heidelberg, Heidelberg, Germany
    (Recipient of the 2016 Lasker-DeBakey Clinical Medical Research Award)
    Hepatitis C virus – Host cell interaction: making the invisible visible
    11:00-11:20 Coffee break
    11:20-12:20 Chiara D'Onofrio Lecture
    Matteo Iannacone, San Raffaele Research Institute, Milan, Italy
    In vivo imaging of antiviral immune responses
    12:20-12:35 Gold supporter talk
    Silvano Spinelli, BiovelocITA
    BiovelocITA in the Italian research environment
    12:35-13:45 Lunch buffet
    13:00-15:00 Poster Session II
    Session III Cell Identity in Health and Disease
    Chairs: Fabiola Moretti, Lucia Falbo
    15:00-15:40 Massimiliano Pagani, Istituto Nazionale Genetica Molecolare-INGM & University of Milan, Milan, Italy
    Interrogating tumoral immune-micro-environment by single cell transcriptomics
    15:40-16:00 Short talk: Giacomo Donati, University of Turin, Turin, Italy
    Hidden potential of differentiated epidermal cells
    16:00-16:20 Short talk: Luigi Fattore, Fondazione G. Pascale, Naples, Italy
    Melanoma cell proliferation and resistance to target therapy is controlled by the reciprocal interplay between MITF and miR-579-3p
    16:20-16:35 Gold supporter talk
    Aida Soler, Sr. Marketing Technical Specialist, EMEA Regional Marketing, Illumina
    Introduction to single cell genomics solution Illumina – BioRad
    16:35-16:50 Gold supporter talk
    Amy Hamilton, Senior Field Applications Specialist, EMEA, Fluidigm Corporation
    The rich world of the single cell
    16:50-17:20 Coffee break
    17:20-18:00 Irene Bozzoni, Sapienza University, Rome, Italy
    From coding to non coding RNAs and back
    18:00-18:45 Armenise Lecture
    Annalisa Di Ruscio, University of Eastern Piedmont, Novara, Italy
    Can we reDiRect epigenetic changes?
    19:00-20:00 SIBBM Society meeting
    20:00-21:30 Buffet dinner
  • Friday, 16 June

    Session IV Genome Landscape and Function
    Chairs: Nicoletta Landsberger, Giovanni Cuda
    8:45-9:25 Epigen Lecture
    Henk Stunnenberg, Radboud University, Nijmegen, The Netherlands
    Systems biology of pluripotent stem cells
    9:25-9:45 Short talk: Samuel Zambrano, San Raffaele Scientific Institute, Milan, Italy
    Synchronous NF-kappaB oscillations translate into functionally related patterns of gene expression
    9:45-10:05 Short talk: Roberta Noberini, Istituto Italiano di Tecnologia, Milan, Italy
    Reprogramming of histone post-translational modifications in cancer model systems
    10:05-10:25 Short talk: Fulvio Chiacchiera, European Institute of Oncology (IEO), Milan, Italy
    Function and mechanism of action of the polycomb repressive complexes in the homeostasis of adult intestinal cells
    10:25-10:40 Gold supporter talk
    Silvia Di Meglio, Scientific Liaison Specialist – Biology, Merck-Sigma-Aldrich
    sygRNA: Synthetic 2 part CRISPR RNA system
    10:40-10:55 Gold supporter talk
    Claudio Comunian, Product Manager Molecular/Cellular Biology Carlo Erba Reagents
    Primary cells in regenerative medicine & tissue engineering
    10:55-11:30 Coffee break
    11:30-12:10 Fabrizio D'Adda di Fagagna, FIRC Institute of Molecular Oncology (IFOM), Milan, Italy
    The role of non coding RNA in chromosomal integrity
    12:10-12:50 Salvatore Oliviero, University of Turin, Turin, Italy
    Regulation and function of DNA methylation
    12:50-13:20 Chiara D’Onofrio junior award (best oral presentation) and "Francesca Martini" prize (best poster)
    Concluding Remarks
  • Posters

    When preparing your poster, please remember that its maximum size is A0 (841 x 1189 mm; width x height). Note that the orientation is portrait, not landscape.

    Correct format... poster should be PORTRAIT  poster should NOT be LANDSCAPE Wrong format...

    Posters will be on display for the entire duration of the meeting. Posters with an even number will be presented during Poster Session I (Wednesday, 14 June). Posters with an odd number will be presented during Poster Session II (Thursday, 15 June).

    P.1 Dario Antonini (Naples)
    P63 and P73 regulate cellular proliferation via distinct mechanisms in cutaneous squamous cell carcinoma

    P.2 Laura Bavagnoli (Pavia)
    The human microRNA miR-1307 is involved in antiviral response and cell proliferation

    P.4 Rebecca Bertolio (Trieste)
    Mechanical cues control mutant p53 stability through a Mevalonate/RhoA axis

    P.5 Andrea Bianchi (Milan)
    The multifaced satellite cells dysfunctions in Emery Dreifuss Muscular Dystrophy

    P.6 Marco Bianchi (Milan)
    How many nucleosomes in mammalian cells?

    P.7 Giulia Callegaro (Milan)
    Image analysis applied to Cell Transformation Assay to advance carcinogenicity testing: morphology evaluation of transformed foci and mitochondria

    P.8 Nicolò Caporale (Milan)
    From cohorts to molecules via organoids: dose-response patterns of endocrine disruption in human neurodevelopmental models

    P.9 Davide Cassani (London, United Kingdom)
    Cryo electron tomography of the actomyosin cortex in isolated blebs

    P.10 Cristina Cheroni (Milan)
    Systems biology approaches to the study of gene networks involved in antiviral interferon response

    P.11 Domenica Cilli (Milan)
    Drug screening of patient-derived cancer organoids

    P.12 Germana Ciolli (Rome)
    Effect of maternal care on murine induced tumors

    P.13 Federica Colombo (Milan)
    NF-κB in motion: role of NF-κB dynamics in multiple myeloma - stromal cells interactions

    P.14 Marco Corazzari (Novara)
    Aldo-keto reductases confer resistance of human skin melanoma to ferroptosis induction

    P.15 Chiara Cordiglieri (Milan)
    Advanced microscopy techniques to decipher neuro-immunological interactions and monitor novel therapies 

    P.16 Riccardo Cristofani (Milan)
    Autophagy mediated HSPB8 removal of aggregating dipeptides produced in C9ORF72 related neurodegenerative diseases

    P.17 Giovanna D'Agostino (Benevento)
    A novel cell stress mechanism related to immunogenic cell death

    P.18 Roberta Dal Magro (Monza)
    Development of a 3D in vitro model mimicking the changes occurring at the blood-brain barrier in Alzheimer’s disease

    P.19 Giulia Della Chiara (Milan)
    Dissecting the dynamic interplay between colorectal cancer and T regulatory cells by exploiting the organoid models

    P.20 Antonina Di Maio (Pavia)
    The Splicing Program of SRSF1transcripts is finely tuned by cell metabolism

    P.21 Anna Di Matteo (Pavia)
    A hierarchy of splicing factors integrating splicing decisions during angiogenesis

    P.22 Raffaella Di Micco (Milan)
    The role of DNA damage response in hematopoietic stem and progenitor cells biology upon gene-targeting

    P.23 Stella Donato (Naples)
    G6PD an oxidative stress defence enzyme essential for correct neuronal differentiation

    P.24 Ira Espuny-Camacho (Leuven/Milan)
    Hallmarks of Alzheimer’s disease in stem-cell- derived human neurons transplanted into mouse brain

    P.25 Francesco Ferrari (Milan)
    Transcriptional up-regulation and conformation differences in D. melanogaster dosage compensation of chromosome X

    P.26 Luca Ferrari (Milan)
    Position effect in 17q11.2 deleted region generating the RNF135-SUZ12 chimeric gene in a NF1 microdeletion patient

    P.27 Federica Ferrarini (Verona)
    Characterization of FOXP2-Δ11, a novel alternatively spliced product of FOXP2 gene

    P.28 Stefania Fochi (Verona)
    Host-virus interactions: HTLV antisense regulatory proteins play a role in the dysregulation of NF-κB pathway

    P.29 Veronica Foletto (Trento)
    Exploring prostate tissue homeostasis and tumorigenesis with 3D organoids

    P.30 Mattia Forcato (Modena)
    Comparison of computational methods for the analysis of Hi-C data

    P.31 Matilde Forcella (Milan)
    Biochemical characterization of cells from transformed foci: a tool for exploiting the Cell Transformation Assay

    P.32 Michele Gabriele (Milan)
    YY1 haploinsufficiency causes an intellectual disability syndrome featuring transcriptional and chromatin dysfunction

    P.33 Maura Galimberti (Milan)
    HD modelling by using iPSC 3D differentiation into cortical domains

    P.34 Giovanni Giuliano (Rome)
    The eggplant genome reveals key events in Solanaceae evolution

    P.35 Daniela Gnani (Milan)
    Non-cell autonomous role of senescent stroma in hematopoietic stem cells aging

    P.36 Aymone Gurtner (Rome)
    Dysregulation of microRNA biogenesis in cancer: the impact of mutant p53 on Drosha complex activity

    P.37 Andrea Iannello (Orbassano (TO))
    Epigenetic changes of genomic regulatory regions in Th17 and Treg cells during pregnancy of Relapsing Remitting Multiple Sclerosis patients

    P.38 Vincenzo Ingangi (Naples)
    Integrating M2-like macrophages into organotypic co-cultures to increase growth and spreading of sarcoma spheroids: a model to test the efficacy of new anti-tumor drugs 

    P.39 Miroslava Kissova (Pavia)
    Alt-EJ: a novel backup pathway in double strand breaks repair orchestrated by pol λ 

    P.40 Mattia Laffranchi (Brescia)
    The novel pathological variant alpha-1-antitrypsin Trento (E75V) shows non-classical behaviour during polymerization

    P.41 Nicoletta Landsberger (Segrate)
    A novel mouse model of Rett syndrome reveals new chromatin phenotypes

    P.42 Patrizia Lavia (Rome)
    Protein SUMOylation at kinetochores: control, functions and in situ visualisation in intact cells

    P.43 Brunno Levone (Cork, Ireland)
    The effects of the antidepressant fluoxetine and the stress hormone corticosterone on neurogenesis on neural progenitor cell (NPC) cultures derived from the dorsal, intermediate and ventral sub-regions of the hippocampus

    P.44 Alessia Loffreda (Milan)
    C-terminal acetylation tunes the p53 single molecule residence time on chromatin in living cells to modulate its transcriptional activity

    P.45 Alessandra Longaretti (Segrate)
    Synapse to nucleus cross-talk and its effect on IEG transactivation in response to stress

    P.47 Mariangela Lorenzo (Milan)
    Functional characterization of lincRNAs in human CD4+ regulatory T lymphocytes

    P.48 Valeria Lucchino (Catanzaro)
    Zinc finger protein 521 (ZNF521) co-operates with Gli factors and enhances the Hedgehog signalling pathway activity

    P.49 Ludovico Maggi (Pisa)
    Engineered virus-like particles for catching dendritic RNAs

    P.50 Alessandro Maglione (Turin)
    Integrative analysis of omics data reveals ERα-modulated core genomic regions in Th17 and Treg cells

    P.51 Sagar Malhotra (Trento)
    A brief review of effects of 3D environment on drug efficiency

    P.52 Cecilia Mannironi (Rome)
    miR-135a regulates synaptic transmission and anxiety-like behavior in amygdala

    P.53 Marina Mapelli (Milan)
    Oligomeric Inscuteable:LGN complexes revert p53-null breast stem cell expansion by promoting Inscutable-mediated asymmetric cell divisions

    P.54 Federica Marasca (Milan)
    Epigenetic role of DNA Transposable Elements (TEs) in shaping CD4+ T cell identity and plasticity

    P.55 Jean Piero Margaria (Turin)
    Activation of Rab11 on peripheral endosomes induces MTM1-mediated PtdIns(3)P depletion required for recycling cargo transport to the peri-nuclear compartment

    P.56 Valeria Mariotti (Padua)
    Establishment and expansion of functional polarized monolayers and 3D tubular structure of cholangiocytes derived by induced pluripotent stem cells (iPSCs) for human biliary disease modeling

    P.57 Enzo Martegani (Milan)
    Detection of PKA activity in budding yeast, using  a FRET-based A-kinase activity reporter 

    P.58 Monica Marzagalli (Milan)
    Single cell autofluorescence as a marker of melanoma stem cells

    P.59 Elisa Mazzoni (Verona)
    Osteogenicity and bone re-growth induced by an innovative collagen/hydroxylapatite hybrid scaffold: studies in vitro and in vivo

    P.60 Giorgio Merlo (Turin)
    Hyper-active GTPase Rac, due to loss of the negative regulator ArhGAP15, affects neuritogenesis and neuronal migration via altered actin dynamics

    P.61 Rosanna Mezzapelle (Milan)
    Malignant mesothelioma organoids, mesospheres and patient derived xenografts as tools to study drug sensitivity and tumor microenvironment

    P.62 Rohini Nair (Milan)
    Histone flow from the nucleus to extracellular vesicles

    P.63 Monica Nizzardo (Milan)
    Modelling Spinal Muscular Atrophy with human 3D culture systems for pathogenic studies and therapeutic development

    P.64 Monica Oldani (Milan)
    Biochemical characterization of cells from transformed foci: a tool for exploiting the Cell Transformation Assay

    P.65 Stefania Oliveto (Milan)
    The oncogenic role of polyribosome-associated miR24-3p in Malignant Pleural Mesothelioma

    P.66 Ivana Ollà (Pisa)
    Development of a CST-based in vitro screening to fish therapeutic molecules for Alzheimer disease 

    P.67 Ivan Orlandi (Milan)
    Resveratrol supplementation at the diauxic shift increases oxidative stress and reduces yeast Chronological LifeSpan

    P.68 Francesca Parolini (Verona)
    CRISPR/Cas9 to study virus-host interactions

    P.69 Angela Perri (Catanzaro)
    Proteomic approach to profile novel histone post translational modifications in breast cancer and to assess their role in carcinogenesis process

    P.70 Mariateresa Pettinato (Milan)
    The immunophilin FKBP12 regulates BMP receptor responsiveness, linking iron homeostasis and inflammation

    P.71 Giulia Piaggio (Rome)
    In vivo imaging of macro-environment before tumor appearance: a novel approach to identify early tumor biomarkers

    P.72 Ernesto Picardi (Bari)
    RNA editing heterogeneity in human brain revealed by Single Cell RNAseq

    P.73 Eleonora Piscitelli (Segrate)
    Characterization of mammary cells derived from single cells with stem cell properties and organoid formation capacity by cell lineage tracing and cell tracking

    P.74 Christian Pistore (Busto Arsizio)
    DNA methylation variations are required for epithelial-to-mesenchymal transition induced by cancer-associated fibroblasts in prostate cancer cell

    P.75 Davide Pradella (Pavia)
    Nova2 alternative splicing regulation of the Netrin receptor Unc5b during angiogenesis

    P.76 Alfredo Procino (Naples)
    The culture medium conditioned by adipose tissue, is able to regulate the lncRNA HOTAIR, HOXC11, HOXC12, Osx and SATB2 gene expression in MSCs during osteoblasts differentiation

    P.77 Manuela Quintavalle (Rozzano (MI))
    Identification of UHRF1 as a new epigenetic regulator of smooth muscle cell-driven vascular pathologies

    P.78 Lorena Quirico (Turin)
    Axl-miR-based sequence chimeric aptamers for targeted cancer therapy 

    P.79 Valentina Riva (Pavia)
    New insights into DDX3 roles: from RNA viruses to RNA metabolism of human normal and tumor cells

    P.80 Ilaria Romito (Rome)
    Effects of FAK silencing/inhibition on liver cancer stem cells markers in HCC cell lines

    P.81 Lorenza Ronfani (Milan)
    Generation of genetically modified mice using the CRISPR-Cas9 technology

    P.82 Valeria Rosato (Benevento)
    miR-27a is a master regulator of colorectal cancer cells metabolism

    P.83 Francesca Rossi (Rome)
    Molecular analysis of the role of a circular RNA in myoblast proliferation and in rhabdomyosarcoma

    P.84 Martina Rossi (Bologna)
    A novel three dimensional co-culture model of hAECs and MSCs

    P.85 Badrunnesa Urmi Sheikh (Rome)
    Lymphoblastoid cell lines as a tool in the study of molecular mechanism of Diamond Blackfan anemia

    P.86 Monica Soldi (Milan)
    Chromatin proteomics reveals that the novel combinatorial histone marks H3K4me1/K36me2/3 and H3K4me1/K79me2 discriminate different classes of macrophage enhancers

    P.88 Antonio Totaro (Padua)
    YAP/TAZ link cell mechanics to Notch signalling to control epidermal stem cell fate

    P.89 Lorena Urbanelli (Perugia)
    Lipid analysis of extracellular vesicles released by fibroblasts undergoing H-Ras induced senescence unravels a potential mechanism to stimulate lysophosphatidic receptor signaling in recipient cells

    P.90 Tania Velletri (Milan)
    Generation and molecular characterization of monoclonal organoids from serous ovarian cancer

    P.91 Megi Vllahu (Fisciano (SA))
    TFF1 regulation from acute to chronic inflammatory process during Helicobacter infection

    P.92 Samuel Zambrano (Milan)
    Stochastic transcriptional response to inflammatory stimuli

    P.93 Matteo Zanella (Milan)
    BAZ1B dosage controls the transcriptional and epigenetic program of neural crest stem cells in two symmetric neurodevelopmental disorders